Biological Classification And System Of Classification Question 552
Question: Mycoplasma can be cultured on
Options:
A) P.D.A. medium
B) C.P.A. medium
C) Hayflick’s (modified) medium
D) None of these
Show Answer
Answer:
Correct Answer: C
Solution:
Mycoplasma, including species such as Mycoplasma pneumoniae, can be cultured on Hayflick’s (modified) medium.
This medium was originally devised by Edward and later modified by Hayflick. The essential and major nutrient constituents of this medium are beef heart infusion, peptone, fresh yeast extract, and unheated horse serum.
Mycoplasma species are fastidious and grow slowly in culture media. They require cholesterol or sterol and nucleic acid precursors for growth. Human or horse serum provides cholesterol and fatty acids, which are required for the synthesis of the cell membrane, as these bacteria are unable to synthesize the components of the cell membrane by themselves.
Therefore, the correct answer is option C: Hayflick’s (modified) medium.
BETA
