Biotechnology-Principles-And-Processes Question 149

Question: Recombinant DNA can be transferred into host cell by:

Options:

A) growing the host cell in growth medium containing ampicillin.

B) coating the DNA with carbohydrates so that the cells will engulf the DNA.

C) treating cells with calcium ions or electrical pulses to increase cell permeability.

D) injecting proteins into host cells to make them more permeable.

Show Answer

Answer:

Correct Answer: C

Solution:

[c] Treating cells with ampicillin does not make them more permeable to DNA.

Coating DNA with lipids (not carbohydrates) is used to introduce recombinant DNA molecules into host cells, injecting DNA (not protein) is a method used to place recombinant DNA into best cells.