Biotechnology Principles And Processes Question 40
Question: Match column-I with column-II and choose the correct option.
Column-I Column-II A. Eco RI I. Bacillus amyloliquefaciens B. Bam HI II. Haemophilus influenza C. Hind III III. Escherichia coli D. pBR 322 IV. Artificial plasmid
Options:
A) A-III; B-I; C-II; D-IV
B) A-II; B-I; C-IV; D-III
C) A-IV; B-I; C-III; D-II
D) A-I; B-IV; C-II; D-III
Show Answer
Answer:
Correct Answer: A
Solution:
[a] A: Eco RI is an endonuclease enzyme isolated from strains of E.
coli, and is part of the restriction modification system.
B : Barn HI (from Bacillus amyloliquefaciens) is a type II restriction endonuclease having the capacity for recognising short sequences of DNA and specifically cleaving them at a target site.
C: Hind III is a type II site-specific deoxyribonuclease restriction enzyme isolated from Haemophilus influenzae that cleaves the DNA palindromic sequence AAGCTT in the presence of the cofactor $ M{g^{2+}} $ via hydrolysis.
D: pBR 322 is a plasmid and was one of the first widely used E.
coli cloning vectors.
BETA
