Cell The Unit Of Life Question 24

Question: Arrange the following steps in a correct sequence as per Gram’s staining technique.

(i) Treatment with 0.5% iodine solution. (ii) Washing with water. (iii) Treatment with absolute alcohol/acetone. (iv) Staining with weak alkaline solution of crystal violet. The correct sequence is:

Options:

A) (ii), (i), (iv), (iii)

B) (iv), (i), (ii), (iii)

C) (iii), (ii), (i), (iv)

D) (iv), (iii), (ii), (i)

Show Answer

Answer:

Correct Answer: B

Solution:

[b] Steps of Gram’s staining technique.

(i) Staining with weak alkaline solution of crystal violet.

(ii) Treatment with 0.5% iodine solution.

(iii) Washing with water.

(iv) Treatment with absolute alcohol/acetone.