Molecular Basis Of Inheritance Question 118
Question: If a nucleotide lacking a hydroxyl group at the 3’ end is added to a PCR, what would be the outcome?
Options:
A) No additional nucleotides would be added to a growing strand containing that nucleotide.
B) Strand elongation would proceed as normal.
C) Nucleotides would only be added at the 5’ end.
D) T. aquaticus DNA polymerase would be denatured.
Show Answer
Answer:
Correct Answer: A
Solution:
[a] A hydroxyl group at the 3’ position of a nucleotide is necessary for the binding of any additional nucleotides.
If this hydroxyl group were absent, no other nucleotides could be added to a growing strand.
BETA
