Biomolecules Question 200
Question: A mixture of two amino acids having pI 9.60 and 5.40 can be separated
Options:
A) by adjusting the pH of the solution at 9.60
B) by adjusting the pH of the solution at 4.20
C) by adjusting the pH of the solution at 7.0
D) by adjusting the pH of the solution at 7.5.
Show Answer
Answer:
Correct Answer: A
Solution:
[a] Every amino acid exists exclusively as dipolar ion when the pH of the solution is equal to its isoelectric point (pI), hence at this pH it does not migrate to either electrode, while at other pH, an amino acid migrates either to cathode or to anode depending upon its pI.
Thus at pH 9.60, amino acid with pI 5.40 will exist as an anion and migrate to anode; while that with pI 9.60 will not migrate to any electrode.
BETA
