Polymerase Chain Reaction

Polymerase Chain Reaction

graph TB PCR(Polymerase Chain Reaction) PCR --> DNA[DNA Denaturation] PCR --> Primer[Primer Annealing] PCR --> Extension[Extension/elongation] DNA --> T1[Temperature: 94-98°C] Primer --> T2[Temperature: 50-65°C] Extension --> T3[Temperature: 72°C] T1 --> T1a[Breaks hydrogen bonds] T2 --> T2a[Primers bind to target DNA] T3 --> T3a[DNA polymerase synthesizes new DNA]


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