Steps In Gram Staining

Steps in gram staining

graph TD; A[Steps in Gram Staining]; A --> B[Step 1: Crystal Violet]; A --> C[Step 2: Iodine Treatment]; A --> D[Step 3: Alcohol Wash]; A --> E[Step 4: Safranin Counterstain]; B --> F[Stains cell wall purple]; C --> G[Binds to crystal violet and forms a complex]; D --> H[Decolorizes cells]; E --> I[Stains decolorized cells pink]; F --> J[All bacteria]; G --> K[All bacteria]; H --> L[Gram-positive bacteria retain color]; H --> M[Gram-negative bacteria lose color]; I --> N[Gram-negative bacteria];


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