Biotechnology Principles And Processes Ques 1

1. Match the organism with its use in biotechnology.

[2020]

(a) Bacillus (i) Cloning vector thuringiensis

(b) Thermus (ii) Construction aquaticus of first rDNA molecule

(c) Agrobacterium (iii) DNA tumefaciens polymerase

(d) Salmonella (iv) Cry proteins typhimurium

Select the correct option from the following:

$ \begin{array}{lllll} & \text { (A) } & \text { (B) } & \text { (C) } & \text { (D) } \\ \text { (a) } & \text { (iv) } & \text { (iii) } & \text { (i) } & \text { (ii) } \\ \text { (b) } & \text { (iii) } & \text { (ii) } & \text { (iv) } & \text { (i) } \\ \text { (c) } & \text { (iii) } & \text { (iv) } & \text { (i) } & \text { (ii) } \\ \text { (d) } & \text { (ii) } & \text { (iv) } & \text { (iii) } & \text { (i) } \end{array} $

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Answer:

Correct Answer: 1.( a )

Solution:

  1. (a) Bacillus thuringiensis is a source of Cry- proteins. Thermus aquaticus is a source of thermostable DNA polymerase (Taq polymerase) used in PCR. Agrobacterium tumefaciens is a cloning vector. The construction of $1^{s t}$ recombinant DNA molecule was performed using native plasmid of Salmonella typhimurium.