Biotechnology Principles And Processes Ques 58
58. Biolistics (gene-gun) is suitable for
[2012M]
(a) DNA fingerprinting.
(b) Disarming pathogen vectors.
(c) Transformation of plant cells.
(d) Constructing recombinant DNA by joining DNA fragments with vectors.
Show Answer
Answer : c
Hints & Solutions
(c) Biolistic is a direct gene transfer method for constructing recombinant DNA. The gene gun was invented by John C. Sanford with Edward Wolf. A gene gun can be used to genetically transform cells or whole organisms with foreign DNA by aiming the barrel of the gun and firing. The microprojectiles in the biolistic gene gun are made of microscopic (or nano) sized gold or platinum powders. These expensive powders are soaked in DNA or RNA (in raw or plasm, form) that are engineered for insertion into the genome of the cells or organisms under the gun.
BETA
