Biotechnology Principles And Processes Ques 8
8. The correct order of steps in Polymerase Chain Reaction (PCR) is
(a) Extension, Denaturation, Annealing
(b) Annealing, Extension, Denaturation
(c) Denaturation, Annealing, Extension
(d) Denaturation, Extension, Annealing
Show Answer
Answer : c
Hints & Solutions
(c) PCR is based on three simple steps required for any DNA synthesis reaction: (i) denaturation of the template into single strands; (ii) annealing of primers to each original strand for new strand synthesis; and (iii) extension of the new DNA strands from the primers.
BETA
