Evolution Ques 80
80. Using imprints from a plate with complete medium and carrying bacterial colonies, you can select streptomycin resistant mutants and prove that such mutations do not originate as adaptation. These imprints need to be used
[2005]
(a) on plates with and without streptomycin
(b) on plates with minimal media
(c) only on plates with streptomycin
(d) only on plates without streptomycin
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Answer : c
Hints & Solution
(c) A number of sterile agar plates having antibiotic streptomycin were prepared. Bacteria were inoculated on the streptomycin plates from the master plate by sterile velvet plate. Most bacterial colonies did not form replicas and inoculum died. Moreover a few colonies survived showing that they were resistant to streptomycin.
BETA
