Molecular Basis Of Inheritance Ques 45
45. Binding of specific protein on regulatory DNA sequence can be studied by means of [1993]
(a) ultra centrifugation
(b) electron microscope
(c) light microscope
(d) X-ray crystallography
Show Answer
Answer : d
Hints & Solution
(d) X-ray crystallography is a technique to study the binding of specific proteins to regulatory DNA. X-ray crystallography or single-crystal X-ray diffraction is an analytical technique which uses the diffraction pattern produced by bombarding a single crystal with X-rays to solve the crystal structure. The diffraction pattern is recorded and then analyzed or “solved” to reveal the nature of the crystal. This technique is widely used in chemistry and biochemistry to determine the structures of an immense variety of molecules, including inorganic compounds, DNA, and proteins.
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