Biomolecules Ques 36
36. A reagent suitable for the determination of $N$-terminal residue of a peptide is
[1996]
(a) $p$-Toluenesulphonyl chloride
(b) 2,4-Dinitrophenylhydrazine
(c) Carboxypeptidase
(d) 2,4-Dinitrofluorobenzene
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Answer:
Correct Answer: 36.(d)
Solution:
(d) 2,4-Dinitroflurobenzene, also known as Sanger’s reagent, reacts with the $H_2 N$ - group of the peptide to from 2, 4- dinitrophenyl (DNP) derivative of the peptide. The DNP derivative of the peptide is hydrolysed to give DNP derivative of the single amino acid
BETA
