Biotechnology Principles And Processes Part 5

RECOMBINANT DNA TECHNOLOGY STEPS#

1. Genetic Material Isolation

• Extract DNA from source organism (donor)
• Purify DNA using specialized techniques

2. DNA Manipulation

• Cut DNA using restriction enzymes
• Join DNA fragments using DNA ligase

3. DNA Insertion and Expression

• Insert recombinant DNA into host cells
• Allow host cells to express desired genes

4. Large-scale Production

• Grow modified cells in bioreactors
• Extract and purify final product