Pyq- Biotechnology Principles And Processes Part 3 L-4

Plasmid pBR322 has a PstI restriction enzyme site within gene lacZ that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactosidase production and the recombinant plasmid is inserted into an E. coli strain, (NEET-2021)

A) it will lead to the lysis of host cells.

B) it will be able to produce a novel protein with dual abilities.

C) it will not be able to confer ampicillin resistance to the host cell.

D) the transformed cells will have the ability to resist ampicillin as well as produce β-galactosidase.

Answer: it will not be able to confer ampicillin resistance to the host cell.



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